Primer of Diagnostic Electron Microscopy
for Pathologists-in-training

Published by

Pathology Images Inc. &
Society for Ultrastructural Pathology
ISBN 0-9736518-0-6
An aid in basic interpretation of electron micrographs to:
  • assist in preparing for examinations in pathology;
  • act as a resource for recently qualified pathologists to acquire experience in electron microscopy
This practical instructional manual for pathology residents/registrars and recently qualified pathologists has been out of print for some years. In this CD-ROM version, the basic layout of the original printed version and electron microscopic images have been retained, but text and images have been updated, new sections added and the number of EM diagnostic entities expanded.
Each CD-ROM includes a 12-page booklet explaining how to use the CD and its content, and maximize viewing the many EM images.
 Ordering Information


The CD-ROM can be ordered on line at:
A link to the Table of Contents and sample pages for review are available on this web page.

Other pathology-related publications authored by members of the Society for Ultrastructural Pathology

Feroze Ghadially Ultrastructural Pathology of the Cell and Matrix
Brian Eyden — Organelles in Tumor Diagnosis: An Ultrastructural Atlas
Robert Erlandson — Diagnostic Transmission Electron Microscopy of Tumors
Lauren Langford — Human Brain Cancer (CD-ROM)
Claire Payne — Electron microscopy in the Diagnosis of Infectious Diseases
Irving Dardick — Color Atlas/Text of Salivary Gland Tumor Pathology
John Hicks — Ewing's Sarcoma Family of Tumors

Electron Microscopy Publications

Leong AS-Y, Wick MR, Swanson PE: Immunohistology and Electron Microscopy of Anaplastic and Pleomorphic Tumors
The Science of Biological Specimen Preparation for Microscopy, Edited by Malecki M, Roomans GM

Diagnostic Ultrastructural Pathology:
A Self-Evaluation and Self-Teaching Manual

Feroze N. Ghadially

Published by: Butterworth-Heinemann, Boston

2nd edition, 1998 - US $35.00 / £19.99

This practical book has been out of print for almost a decade. The new edition remains faithful to the principles of the initial work, an excellent resource for teaching and self-leaning diagnostic electron microscopy, but is about 50 percent larger. An appendix has also been added with line drawings that aid in understanding some of the more difficult to understand topics in the book. As Professor J.M. Nesland states in a review in Ultrastructural Pathology, "This book is especially easy to read and can be highly recommended for young residents and people subspecializing in diagnostic EM.".

Ultrastructural Pathology of the Cell and Matrix,

Feroze N. Ghadially

Published by: Butterworth-Heineman, Boston

4th edition, 2 volumes 1414 pp, 1997

Book Review by Ivan Damjanov:
Modern Pathology, March 1998, Vol. 11, page 305

About this tome, Dr. Damjanov says in part: "His book remains essential reading for all those entering the field, but it is also indispensable to all those who use electron micrsocopy for either research or diagnosis. I should add also that for those who use electron microscopy for teaching, it is still one of the best ways to teach students to see through the ordinary light microscope."

The latter truism does not receive enough attention from surgical pathologists.


Brian Eyden, BSc, PhD

Clinical Scientist and Head of Electron Microscopy, Department of Histopathology, Christie Hospital National Health Service Trust, Manchester, England

Distributed by:
Williams & Wilkins
351 West Camden Street
Baltimore, Maryland

Designed for daily use at the microscope, this book is a new, practical atlas that will enable pathologists to accurately identify diagnostic cell structures and tumors through the use of electron microscopy. The atlas has been especially designed to demonstrate to the pathologist how electron microscopy can be used as a tool for reporting accurate descriptions and interpretations of tumors. This is accomplished using 300 carefully selected micrographs of diagnostically important tumor cell organelles and detailed but concise descriptive text that clearly describes tumor cell organelles to aid the pathologist in identifying them as distinctive cell structures

1996, 192 pgs., 79 plates of photos — LC: 96-1857, ISBN: 0-89640-311-4
$59.00 (New lower pricing)
To order, call toll free 1-800-638-0672

DIAGNOSTIC TRANSMISSION ELECTRON MICROSCOPY OF TUMORS with Clinicopathological, Immunohistochemical, and Cytogenetic Correlations

Robert A. Erlandson, PhD

Attending Electron Microscopist, Department of Pathology, Memorial Sloan-Kettering Cancer Center and Associate Professor of Pathology, Cornell University Medical College

This text book is a compendium on the subject that presents pathologists and scientists with concise, up-to-date information about the ultrastructure of human tumors. It is based on the examination of over 6,500 human tumors over many years and a critical review of the literature. It is the current standard for diagnostic electron microscopy in the field of human tumor pathology.

Published by:
Raven Press, Ltd.,
1185 Avenue of the Americas,
New York, NY 10036
1994, 857 pgs. LC: 93-1632, ISBN: 0-7817-0042-6

HUMAN BRAIN CANCER: Diagnostic Decisions

Lauren A. Langford, MD, Dr. med

Assistant Professor of Neuropathology, Department of Pathology, The University of Texas M.D. Anderson Cancer Center, Houston Texas

Distributed (US) by:
SilverPlatter Education Inc., 246 Walnut Street, Suite 302 Newton, MA 02118

Tel: 617-244-0284 (ext. 15)

This exceptional CD-ROM effectively uses a multimedia approach with a combination of detailed animations, nearly 500 high-quality clinical photographs, and over 200 full-color and black-and-white drawings to demonstrate how to microscopically differentiate normal cellular components from neoplastic cellular components in central nervous system biopsies from primary and metastatic CNS neoplasms.

Distributed (Canada) by:
IHS/Micromedia Ltd.
phone: 416-362-5211 (toll free 1-800-387-2689)

CD-ROMs companion WWW site,


Claire M. Payne, PhD

Research Professor, Department of Microbiology/Immunology, College of Medicine, University of Arizona, Tucson, Arizona

Authored Chapter 2 (volume 1) entitled "Electron Microscopy in the Diagnosis of Infectious Diseases"

Editors: Connor DH, Chandler FW et al.,
Published by: Appleton & Lange, Stamford, CT., 1997.

This chapter provides an overview of the instances in which EM is most helpful in the specific diagnosis of infectious diseases. An understanding of the ultrastructural and diagnostic features of certain viruses, bacteria, fungi, and

protozoa, and their clinical settings, can have a significant impact on patient care. EM is the best catchall technique that does not rely on any a priori suspicions of the possible etiologic agent. In addition to a diagnostic role, transmission EM may be valuable for proper therapy and prognosis and in avoiding additional and unnecessary invasive procedures for clinical workup, especially in neonates.

The following organisms are discussed in detail:
1) Viruses: herpesviruses, adenovirus, papovavirus, poxvirus, viral agents of gastroenteritis, HIV
2) Parasites of the Subkingdom Protozoa: Toxoplasma, Cryptosporidia, Cyclospora, Leishmania, Giardia, Microsporidia
3) Parasites of Uncertain Taxonomy: Pneumocystis carinii
4) Bacterial Infections: Instances in which EM is helpful: Demonstration of Capsules using Ultrastructural Cytochemistry (certain Bacteroides species), Epidemic Outbreaks of Unknown Cause (Legionella pneumophila), following the therapy for Whipple's Disease


Irving Dardick, MD

Professor of Pathology and Otolaryngology, University of Toronto, Toronto, Ontario

Distributed by: Williams & Wilkins, 351 West Camden Street, Baltimore, Maryland 21201-2436

The scope of this book is limited to the histological diagnosis of salivary gland tumors. The main goals are to detail diagnostic criteria for the various benign and malignant tumors and to provide appropriate and comprehensive illustrations (661 photographs of which 600 are in full color) to assist pathologists in categorizing salivary gland tumors.

1996, 288 pages. LC: 95-26461, ISBN: 0-89640-308-4

To order, call toll free 1-800-638-0672


M. John Hicks, MD, PhD

3rd Edition,
Editors Pizzo, PA and Poplack DG
Lippincott-Raven Publishers, 1997

Chapter 31: Ewings Sarcoma Family of Tumors: Ewings Sarcoma of Bone and Soft Tissue and the Peripheral Primitive Neuroectodermal Tumors
ME Horowitz, MW Malawer, SY Woo, MJ Hicks

This chapter on Ewings Sarcoma family of tumors reviews the epidemiology, molecular features, biology, histopathology, immunocytochemistry, ultrastructure, treatment and prognosis. This tumor family ranges from the classic typical Ewings Sarcoma to the peripheral primitive neuroectodermal tumors. It also discusses a relatively new entity, Biphenotypic Sarcoma, which shares features of Ewings sarcoma and rhabdomyoblastoma while expressing molecular markers characteristic for Ewings sarcoma family of tumors.


Leong AS-Y, Wick MR, Swanson PE

Cambridge, England, Cambridge University Press ,1997

287 pages, $120.00 (US)

Reviewed by Juan P. Palezzo in Modern Pathology, February 1998, vol. 11, page 154.

Dr. Palezzo says in part:
"This book presents the current applications of electron microscopy and immunohistochemistry in the diagnosis of this group of tumors." 

A wide variety of tumors are discussed in detail throughout the book, including sarcomatoid carcinomas, small round cell tumors and those with spindle cell morphology. Each tumor's histologic, immunohistochemical, and ultrastructural features are presented in detail, along with examples illustrated with black-and-white photographs.


Edited by Marek Malecki and Godfried M. Roomans

Proceedings of the 14th Pfefferkorn Conference, Belleville, IL

ISSN: 0892-953X / ISBN: 0-931288-49-5

Hardbound book with 31 papers; 466 + xii pages. Price: $95 (US delivery by uninsured mail) or $105 (elsewhere by uninsured mail); insured delivery, air mail, etc., are available for additional cost, please inquire.

Published by and available from:
Scanning Microscopy Intl., Box 66507, AMF O'Hare (Chicago), IL 60666-0507,

FAX: (847) 985-6698